Genos offers high-throughput analyses with relative quantification of N-glycans and N-glycopeptides developed and optimized for various human and rodent sample types. N-glycans are released from glycoproteins by digestion with PNGaseF, fluorescently labelled with 2-AB, procainamide or APTS, purified and analysed by either hydrophilic interaction ultra-high-performance liquid chromatography with fluorescence detection (HILIC-UHPLC-FLD) or capillary gel electrophoresis with laser-induced fluorescence (CGE-LIF). N-glycopeptides are obtained from glycoproteins by trypsin digestion, purified and analysed by C18 liquid chromatography coupled to mass spectrometry via electrospray ionization (C18-LC-ESI-MS).

Analysis of total released human serum/plasma N-glycans by HILIC-UHPLC-FLD

Analysis of total released human IgG N-glycans by HILIC-UHPLC-FLD

Analysis of subclass-specific human IgG Fc N-glycopeptides by C18-LC-ESI-MS

Analysis of total released human IgA N-glycans by HILIC-UHPLC-FLD

Analysis of subclass-specific human IgA Fc N-glycopeptides by C18-LC-ESI-MS

Analysis of human AGP N-glycopeptides by C18-LC-ESI-MS

Analysis of human C3 N-glycopeptides by C18-LC-ESI-MS

Analysis of total released mouse IgG N-glycans by HILIC-UHPLC-FLD or CGE-LIF

Analysis of subclass-specific mouse IgG N-glycopeptides by C18-LC-ESI-MS

Analysis of subclass-specific rat IgG N-glycopeptides by C18-LC-ESI-MS